Case study

MINIMAL CSF INPUTS FOR REPEATED MIRNA BIOMARKER SAMPLING IN RAT

CHALLENGE: Process establishment for repeated sampling with minimal sample inputs from rat and human CSF samples

SITUATION:

Client: Pharma
Sample: CSF with human and rat origin
Subject: Repeated sampling of rat CSF miRNA biomarkers

Actions


  • Consultation on study setup and sampling
  • Quality assessment of RNA samples
  • Hummingbird-expert quantitation, QC etc.
  • Titration & comparison of different sample inputs
  • Detailed QC report and defined input for high-throughput microarray
  • Bioinformatics analysis

Results


  • Comprehensive raw data & Hummingbird‘s comprehensive Bioinformatics report (incl. publication ready figures, tables)
  • Solution for study design/setup
  • Comprehensive raw data
  • Hummingbird’s comprehensive Bioinformatics report (incl. publication ready figures, tables)
  • Establishment of a validated, proprietary SOP for low sample inputs of CSF