Case study

mrna-profiling for optimization of biotechnological production


CHALLENGE: Identification of production relevant pathway deregulation



SITUATION:

Client: Biotech
Sample: Bacteria
Subject: mRNA-profiling for optimization of biotechnological production

Actions


  • Quality assessment of bacterial RNA samples
  • Hummingbird-expert quantitation, QC etc.
  • Detailed QC report
  • Defined input for microarray
  • Genome-wide transcriptional profiling
  • Bioinformatics analysis

Results


  • Comprehensive raw data & Hummingbird’s comprehensive Bioinformatics report (incl. publication ready figures, tables)
  • Comparison of different fermentation conditions & production constructs strains
  • Quantitation of customer’s target mRNA
  • Confirmed overexpression of customer’s production target
  • Influence of overexpression on cellular metabolism & pathways
  • Comprehensive analysis of pathways & gene ontology enrichment